Gene description for ACTB
Gene name actin beta
Gene symbol ACTB
Other names/aliases BKRNS
BNS
BRWS1
CSMH
DDS1
PS1TP5BP1
THC8
Species Homo sapiens
 Experiment description of studies that identified ACTB in Oral cancer cells

1
Experiment ID1374
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33144925   
OrganismHomo sapiens
Experiment descriptionTriple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer
AuthorsOno K, Sogawa C, Kawai H, Tran MT, Taha EA, Lu Y, Oo MW, Okusha Y, Okamura H, Ibaragi S, Takigawa M, Kozaki KI, Nagatsuka H, Sasaki A, Okamoto K, Calderwood SK, Eguchi T.
Journal name J Extracell Vesicles
Publication year2020
SampleOral cancer cells
Sample nameHSC-3
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID1375
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33144925   
OrganismHomo sapiens
Experiment descriptionTriple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer
AuthorsOno K, Sogawa C, Kawai H, Tran MT, Taha EA, Lu Y, Oo MW, Okusha Y, Okamura H, Ibaragi S, Takigawa M, Kozaki KI, Nagatsuka H, Sasaki A, Okamoto K, Calderwood SK, Eguchi T.
Journal name J Extracell Vesicles
Publication year2020
SampleOral cancer cells
Sample nameHSC-3-M3
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID1376
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33144925   
OrganismHomo sapiens
Experiment descriptionTriple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer
AuthorsOno K, Sogawa C, Kawai H, Tran MT, Taha EA, Lu Y, Oo MW, Okusha Y, Okamura H, Ibaragi S, Takigawa M, Kozaki KI, Nagatsuka H, Sasaki A, Okamoto K, Calderwood SK, Eguchi T.
Journal name J Extracell Vesicles
Publication year2020
SampleOral cancer cells
Sample nameHSC-3-M3
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID1377
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33144925   
OrganismHomo sapiens
Experiment descriptionTriple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer
AuthorsOno K, Sogawa C, Kawai H, Tran MT, Taha EA, Lu Y, Oo MW, Okusha Y, Okamura H, Ibaragi S, Takigawa M, Kozaki KI, Nagatsuka H, Sasaki A, Okamoto K, Calderwood SK, Eguchi T.
Journal name J Extracell Vesicles
Publication year2020
SampleOral cancer cells
Sample nameHSC-3-M3
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID2362
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36748335   
OrganismHomo sapiens
Experiment descriptionHypoxia promotes EV secretion by impairing lysosomal homeostasis in HNSCC through negative regulation of ATP6V1A by HIF-1α
AuthorsWang X, Wu R, Zhai P, Liu Z, Xia R, Zhang Z, Qin X, Li C, Chen W, Li J, Zhang J.
Journal name J Extracell Vesicles
Publication year2023
SampleOral cancer cells
Sample nameHN6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID2363
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36748335   
OrganismHomo sapiens
Experiment descriptionHypoxia promotes EV secretion by impairing lysosomal homeostasis in HNSCC through negative regulation of ATP6V1A by HIF-1α
AuthorsWang X, Wu R, Zhai P, Liu Z, Xia R, Zhang Z, Qin X, Li C, Chen W, Li J, Zhang J.
Journal name J Extracell Vesicles
Publication year2023
SampleOral cancer cells
Sample nameHN6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID2364
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36748335   
OrganismHomo sapiens
Experiment descriptionHypoxia promotes EV secretion by impairing lysosomal homeostasis in HNSCC through negative regulation of ATP6V1A by HIF-1α
AuthorsWang X, Wu R, Zhai P, Liu Z, Xia R, Zhang Z, Qin X, Li C, Chen W, Li J, Zhang J.
Journal name J Extracell Vesicles
Publication year2023
SampleOral cancer cells
Sample nameCAL27
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID2365
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36748335   
OrganismHomo sapiens
Experiment descriptionHypoxia promotes EV secretion by impairing lysosomal homeostasis in HNSCC through negative regulation of ATP6V1A by HIF-1α
AuthorsWang X, Wu R, Zhai P, Liu Z, Xia R, Zhang Z, Qin X, Li C, Chen W, Li J, Zhang J.
Journal name J Extracell Vesicles
Publication year2023
SampleOral cancer cells
Sample nameCAL27
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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